ALL DATA WILL BE GIVEN Must have TITLE ITRODUCTION METHODS RESULTS DISCUSSION AND CITED. Use the steps on the pre lab paper but get exact amount I used of each product from this video. For part 2 lab I did experiment 1 boiling. I’ve written down all the data you need and step by step what I did all you have to do is write it in paragraph format. i have the data and the graph screenshots just be sure to LABEL AND TITLE THE GRAPHS! DUE DATE IS WEDNSDAY 9 P.M EST!!
Enzyme Activity Pre-Lab: Please complete the following BEFORE coming to lab. Submit your
pre-lab to the assignment folder provided before your designated LAB time. Also bring a printed
copy with you to LAB.
1) Define catalyst- Any substance that increases the rate of a reaction without itself
being consumed. (Causing a change)
2) Define enzyme- A substance produced by a living organism which acts as a
catalyst to bring about a specific biochemical reaction.
3) Explain the Importance of Enzymes to physiology- physiology explains how the
human body function and the human body uses a lot of enzymes to function,
knowing how they work in turns helps to know how the human body works.
4) Define reaction Rate- the rate or speed at which a reaction are converted into
5) Define Catalase- An enzyme that brings about (catalyzes) the reaction by which
hydrogen peroxide is decomposed to water and oxygen.
6) Write out the reaction that catalase is responsible for catalyzing- 2H2O2-Ã¢â€ â€™ 2H2O+O2
(Hydrogen peroxide into water and oxygen)
7) Explain why measuring the oxygen concentration can help determine the reaction rate.
Because increasing the concentration can often increase the rate of reaction so
knowing the concentration can help find the rate of reaction.
8) List three factors that could possibly affect catalase activity. Temperature, pH, salt
concentration, and presence of inhibitors or activators
9) Methods: Watch the following Video on how to measure Catalase Activity Using the GO
Direct Oxygen Sensor.
10) Summarize the methods used from the video (Can be in bullet point format).
Ã¢â‚¬Â¢ Launch Graphical Analysis go to sensor data collector and connect go sensor.
Ã¢â‚¬Â¢ Change rate and duration.
Ã¢â‚¬Â¢ Measure hydrogen peroxide out and add to test tube.
Ã¢â‚¬Â¢ Measure out distilled water and add to test tube.
Ã¢â‚¬Â¢ Add your enzyme by using a pipette and add to solution.
Ã¢â‚¬Â¢ Put oxygen gas sensor into bottle.
Ã¢â‚¬Â¢ Start data collection on app.
Ã¢â‚¬Â¢ Analyze data to determine the rate of reaction.
Ã¢â‚¬Â¢ Click on graph tools.
Ã¢â‚¬Â¢ Apply curve fit choose linear and apply.
Ã¢â‚¬Â¢ Slope will give you the initial reaction rate.
Ã¢â‚¬Â¢ Add enzymes and others to see change.
PRELIMINARY ACTIVITY FOR
Testing Enzyme Activity
(Oxygen Gas Sensor)
Guided Inquiry Version
Many organisms can decompose hydrogen peroxide (H2O2) enzymatically. Enzymes are globular
proteins, responsible for most of the chemical activities of living organisms. They act as catalysts,
substances that speed up chemical reactions without being destroyed or altered during the process.
Enzymes are extremely efficient and may be used over and over again. One enzyme may catalyze
thousands of reactions every second.
H2O2 is toxic to most living organisms. Many organisms are capable of enzymatically destroying
the H2O2 before it can do much damage. H2O2 can be converted to oxygen and water as follows:
2 H2O2(aq) Ã¢â€ â€™ 2 H2O + O2(g)
Although this reaction occurs spontaneously, enzymes increase the rate considerably. At least two
different enzymes are known to catalyze this reaction: catalase, found in animals and protists, and
peroxidase, found in plants. A great deal can be learned about enzymes by studying the rates of
In this Preliminary Activity, you will use catalase in yeast to catalytically decompose hydrogen
peroxide. You will use an O2 Gas Sensor to determine the rate of catalase activity by measuring
oxygen gas produced as H2O2 is decomposed.
At the start of the reaction, there is no product, and the O2 concentration is the same as the
atmosphere. Shortly after data collection begins, oxygen accumulates at a rather constant rate. The
slope of the curve at this initial time is constant and is called the initial rate. In this investigation, we
will refer to this as the rate of catalase activity. As the peroxide is decomposed, less of it is
available to react and the O2 is produced at lower rates. When no more peroxide is left, O2 is no
longer produced. When data collection is complete, you will perform a linear fit on the resultant
graph to determine catalase activity.
After completing the Preliminary Activity, you will investigate your assigned researchable
question. Use reference sources to find out more about catalase, enzymes, and enzyme activity
before planning and conducting your investigation.
1. Obtain and wear goggles.
2. Start the data-collection program
Investigating Biology through Inquiry
Ã‚Â©Vernier Software & Technology
6A – 1
3. Connect the O2 Gas Sensor to your Chromebook, computer, or mobile device. Use an
interface if necessary.
4. Prepare to initiate the catalase catalyzed reaction.
a. Use a utility clamp to fasten an O2 Gas Sensor to the Stir Station.
b. Place 10.0 mL of 1.5% H2O2 into a clean 250 mL Nalgene bottle. Take care to minimize
depositing drops on the sides of the bottle.
c. Place a stir bar into the bottle.
5. Initiate the enzyme catalyzed reaction and start data collection. Note: The next steps should be
completed as rapidly as possible.
a. Using micropipette, add 100 Ã‚ÂµL of enzyme suspension to the contents of the Nalgene
b. Swirl the contents of the bottle for 2Ã¢â‚¬â€œ3 seconds to ensure thorough mixing.
c. Place the O2 Gas Sensor into the bottle as shown in Figure 1. Gently push the bottle up
onto the sensor until it stops. Note: The sensor is designed to seal the bottle with minimal
d. Position the O2 Gas Sensor and Nalgene bottle assembly on the Stir Station.
e. Start the magnetic stirrer, and adjust it to a medium speed.
f. Start data collection.
6. When 200 seconds have elapsed, stop data collection.
7. Remove the O2 Gas Sensor from the Nalgene bottle. Rinse the bottle with water and dry it
with a soft paper towel.
6A – 2
Investigating Biology through Inquiry
Testing Enzyme Activity (Oxygen Gas Sensor)
8. Perform a linear fit on the 30Ã¢â‚¬â€œ200 s portion of the graph. Record the slope of the line, m, as the
rate of catalase activity, in % O2/s.
1. What was the rate of catalase activity?
2. Why is it important that cells contain catalase?
3. List three factors that could possibly affect catalase activity.
Investigating Biology through Inquiry
6A – 3
ENZYME ACTION LAB GUIDED INQUIARY PART II
Now that you are comfortable with using both the O2 sensor to determine the rate of
reaction for the breakdown of hydrogen peroxide:
2 H2O2 Ã¯â€šÂ¬
Ã¯â€šÂ¾Ã¯â€šÂ® 2 H2O + O2
It is your turn test what may affect the rate of reaction. Each student at your table
should choose one of the following to test (must be different from other students at the
1) Design an experiment to determine the effect of changing the temperature (Warm to
Boiling) of the catalase on the rate of reaction.
2) Design an experiment to determine the effect of changing the temperature (Cold to
Freezing) of the catalase on the rate of reaction.
3) Design an experiment to determine the effect of changing the enzyme concentration on
the rate of reaction.
4) Design an experiment to determine the effect of changing the amount of substrate on the
rate of the reaction.
5) Design an experiment to determine the effect of changing saline concentration on the rate
of the reaction.
6) Design an experiment to determine the effect of changing the pH concentration on the
rate of the reaction.
Once you have chosen your condition to test, generate a hypothesis stating how you
will think the change will alter the rate of reaction. Remember, a hypothesis is a
prediction you create prior to running an experiment. It states clearly what variables are
being tested, what the predicted outcome will be.
WRITE THIS HYPOTHESIS DOWN BEFORE COMPLETING YOUR EXPERIMENT!
Next, Design an experiment using the materials provided (if you are attending face to
face) and methods. For those attending face to face you should have experimental
design in the form of a general outline stating the materials and methods to be utilized
to test your hypothesis BEFORE collecting your data. Use this outline to complete your
experiment and record your data.
Things to think about in your experimental design:
How many replicates would suffice to help you make accurate conclusions?
How long should you collect the data?
What data will you collect? How is this data going to be represented?
How much of each material will be used?
How will you keep all variable constant except for the one being tested?
What represents your dependent vs. independent variable?
NOTE: For those attending virtually, you are still expected to generate a hypothesis
based off of one of the conditions as well as create an experimental design and write a
Homework: LAB REPORT
Now, that you have collected your data you should be able to determine if you can
reject or accept your hypothesis! Now it is time to generate a lab report. You can
refer to the Guide to writing a Lab Report for general instructions.
All lab reports will require a Title, Introduction, Methods (approach), Results (actual or
predicted), Discussion, and Cited References.
For the Introduction/Discussion you will need to complete background literature
research to help understand your results (if you are attending face to face) or your likely
results (if you are attending virtually). An introduction gives focus to the report similar to
the “Purpose” and/or Ã¢â‚¬Å“ObjectiveÃ¢â‚¬Â. It should put the experiment into context and provide
the reader with information necessary to understand the scientific basis of the
experiment and the techniques used. Typically, the background information provided
should help explain why you formed your hypothesis, however for this lab you did not
have time to complete your background information first (for face to face). So your
background information can be used to explain your results!!!
Remember when writing your discussion the outcome of the experiment will either prove
or disprove your hypothesis. If your results do not match what the literature states then
you may want to think about how you could alter your experimental design.
For the methods be sure to compose a method section in prose/paragraph form. Do not
just copy the step by step bullet points from the Guide to the oxygen sensor. Instead
use your Experimental design outline as a guide.
Results should be presented graphically with a description included that explains what
is shown. You may also include tables if you wish.
NOTE: For those attending virtually, you will be provided with a data set based on
the variable you chose to test.
LAB GROUP 1: Upload your LAB REPORT to the Assignment folder in D2L by
WEDNESDAY FEBRUARY 10 11:59 pm.
LAB GROUP 2: Upload your LAB REPORT to the Assignment folder in D2L by
WEDNESDAY FEBRUARY 17 11:59 pm
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